Coding

Part:BBa_K4202008

Designed by: Hongying LIN   Group: iGEM22_ZJU-China   (2022-09-29)


Amorphous Calcium Carbonate Binding Protein(ACCBP)(with signal peptide)

Amorphous calcium carbonate binding protein(ACCBP) is a protein that can help to form and transform amorphous calcium carbonate(ACC).

In calcium carbonate solution,the ions can automatically aggregate and form prenucleation clusters, which further form ACC through certain pathways. ACC will transform into minerals of different crystal forms. In this process, organisms mainly regulate the biological mineralization process by regulating ACC formation and transformation. ACCBP is an important protein that can help to form ACC and transform ACC into calcite at specific condition.

To ensure that this part can express active protein, the corresponding plasmid should be transformed into the E.coli, and the protein is induced at specific conditions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 189
    Illegal AgeI site found at 452
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 658


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Categories
Parameters
None